Biopsy is generally indicated in the following cases:
A biopsy is not indicated, on the other hand, where the disease is better diagnosed through an electromyogram (EMG) like Myasthenia gravis or Myotonia.
You should wait 1 month after an attack of Rhabdomyolysis.
Biopsy of semitendinosus muscle: site of choice in cases of suspected polysaccharide storage myopathy
In figure 1: Biopsy of sacrocaudal muscle: site of choice in suspected cases of motor neurone disease.
In figure 2: Gluteus: site used for biopsy in suspected cases of immune mediated inflammatory myopathy or in cases of gluteal atrophy.
There are two basic procedures for carrying out a muscle biopsy: the surgical technique known as “open” and the technique using a percutaneous needle, also called punch.
The surgical method with incision into the cutaneous, subcutaneous and fascia layers is preferable to taking a sample using a transcutaneous punch because it allows for a sample which is the right size and direction.
Example of biopsy performed using biopsy needle. This technique is not recommended in veterinary medicine. Source: modified from Dubowitz V, Sewry CA: Muscle Biopsy (third edition), Saunders, Elsevier, 2007
Example of biopsy performed using biopsy needle. This technique is not recommended in veterinary medicine. Source: modified from Dubowitz V, Sewry CA: Muscle Biopsy (third edition), Saunders, Elsevier, 2007
The direction of the muscle fibres in the sample is fundamental as it allows for the correct positioning of the sample during freezing and means that excellent transverse sections can be taken for histomorphological assessment of the muscle tissue.
The biopsy must be performed at the level of the muscle body, avoiding tendon insertion points and aponeuroses where the histology may be different.
The sample needs to be sent to the laboratory with an ice-pack and carefully labelled indicating clinical signs and history.
As an alternative to the containers shown in fig. 1, an ice-pack can be used which is pre-cooled in a freezer and then put in a bag for the transport of biological samples next to the muscle biopsy.
Before collecting the sample, the staff who are going to process the sample at the laboratory should be consulted to make sure what is the best way to collect the sample and to transport it.
1. Introduction to the study of Veterinary Myopathology
2. Structure and characteristics of normal skeletal muscle
2. Structure and characteristics of normal skeletal muscle
3. Diagnosis of neuromuscular pathology
5. Specific alterations to muscle fibres
6. Congenital and hereditary muscle disorders in farm animals
8. Bacterial and Parasitic Myopathies
9. Nutritional and toxic myopathies
10. Polysaccharide storage myopathy